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Image Search Results
Journal: Bio-protocol
Article Title: Optimized Protocol for DNA Extraction in Three Theobroma Species
doi: 10.21769/BioProtoc.5297
Figure Lengend Snippet: (A) Pareto chart showing the DNA concentration (ng/µL) obtained using different extraction protocols (discarded, CTAB, β-mercaptoethanol, and proteinase K). The bars represent the individual contribution of each protocol, while the line indicates the cumulative percentage of extraction efficiency. (B) Comparison of DNA yield between CTAB concentrations (3% and 4%), evaluated by densitometric units on a 1% agarose gel. Asterisks (**) denote statistically significant differences between the two groups. (C) Agarose gel electrophoresis showing the integrity and quality of extracted DNA; (1) 1 kb DNA ladder, (2, 3, 4) DNA from Theobroma cacao , (5, 6, 7) DNA from Theobroma grandiflorum , (8, 9, 10) DNA from Theobroma bicolor . (D) Agarose gel electrophoresis showing DNA from Theobroma cacao subjected to a double CIA extraction step, (1,2) DNA from Theobroma cacao .
Article Snippet:
Techniques: Concentration Assay, Extraction, Comparison, Agarose Gel Electrophoresis
Journal: Genes
Article Title: Rapid Visual Detection of High Nitrogen-Use Efficiency Gene OsGRF4 in Rice ( Oryza sativa L.) Using Loop-Mediated Isothermal Amplification Method
doi: 10.3390/genes14101850
Figure Lengend Snippet: Gel electrophoresis of PCR products produced under different annealing temperatures. Lane M, DNA ladder (MD112, TIANGEN Biotech (Beijing) Co., Ltd., Beijing, China). Lanes 1–8, amplifications at annealing temperatures (Tm) of 56.0 °C, 56.6 °C, 57.7 °C, 59.4 °C, 61.5 °C, 63.2 °C, 64.3 °C, and 65.0 °C, respectively.
Article Snippet: Both PCR and digested products were verified by 3%
Techniques: Nucleic Acid Electrophoresis, Produced